User Questions

UQ1: Characterize the sequence of the plant enzyme Alcohol dehydrogenase. UQ2: Adenylate Kinase is an important enzyme in ATP metabolism. In the area of proteomics you need to get a broad picture of the enzyme. How do you proceed? UQ3: Which enzymes need Selenium as co-factor? UQ4: Enzymes are sorted into Enzyme classes, and a 4 field EC number is assigned (1.2.3.4 represent the enzyme class, subclass, sub-subclass and entry number, respectively). What kind of Enzymes have the number (3. 4. any. any)? UQ5: Searching 2D Gels for dehydrogenases of E. coli. UQ6: In order to study diabetes, you are investigating the proteome of Pancreatic tissue in mice under different experimental conditions. The proteins from pancreas of treated animals were seperated by 2D gel electrophoresis. How can you calibrate your gels?