|
-demo-
You have sequenced the gene of a voltage dependent chloride channel (clc) that is involved in myotonia (Becker's disease and Thomsen's disease), which are characterized by skeletal muscle stiffness as a result of muscle membrane hyperexcitability in human. The sequence was deposited in SwissProt. Can you find it?
- Does the protein have distinct domains?
- How many transmembrane spanning regions does the protein have?
- Are variations of the gene documented?
|
: Open SRS and start a new session by clicking Start.
- Leave the default databases SWISS_PROT and TREMBL checked for your search. Click Continue.
- Enter chloride & channel[Description] into the first search field.
- Enter human[Organism] into the second search field.
- Enter skeletal & muscle[Description] into the third search field.
- Click Do Query.
|
: On the Query Result page,
- Click SWISS_PROT:CLC1_HUMAN for the NiceProt View of SWISS-PROT: P35523.
- View the record for [General] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]
- Cross-references: Discuss your familiarity with the following database:
- MIM: Information on genetic disorders
- Check the cross references for protein domain information.
e.g.,
- ProDom
- BLOCKS
- Pfam (Access to 3D structure for CBS domain)
- At the bottom of the Query Result page you will find a link to more analysis toos as, for example, Direct BLAST submission, ScanProsite and Sequence analysis tools.
|
|
