Adenylate Kinase is an important enzyme in ATP metabolism. In the area of proteomics you need to get a broad picture of the enzyme. How do you proceed? |
| Sample User Question |
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Adenylate Kinase is an important enzyme in ATP metabolism. In the area of proteomics you need to get a broad picture of the enzyme. How do you proceed?
- Which reaction is catalyzed by the enzyme?
- Can you find the reaction on the Boehringer Mannheim "Biochemical Pathways" chart?
- Is there a disease involved in deficiency of adenylate kinase?
- Does Myokinase of human (P00568) also belong to the Adenylate Kinase protein family?
- Starting from the protein signature, find all Adenylate Kinases in Swiss-Prot, TrEmbl and PDB from Eukaryota.
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| Analysis/Comments |
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| Flow Chart |
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- ENZYME - Enzyme nomenclature database
- ScanProsite - (Access from PROSITE home page) Scan a sequence against PROSITE or a pattern against SWISS-PROT or PDB and visualize matches on structures.
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| Step By Step Guide |
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ENZYME
- Search for adenylate kinase using the description search box, click Search.
- Click on 2.7.4.3 from the first results page to view the full entry record.
Entry record 2.7.4.3:
- Reaction catalysed: ATP+AMP <=> ADP+ADP
- Human Genetic Disease(s): Hemolytic anemia due to deficiency of adenylate kinase (MIM:103000)
- Cross-References:
- Biochemical Pathways; map number(s)G7
- PROSITE: Documentation:PDOC00104, Prosite entry:PS00013
ScanProsite
- Access the program from the PROSITE db page or the Proteomics Tools page (Pattern and profile searches).
- Under Search SWISS-PROT with a PROSITE entry enter the PROSTITE pattern ([LIVMFYWCA]-[LIVMFYW](2)-D-G-[FYI]-P-R-x(3)-[NQ]) or PROSITE ID number (PS00013), NOT the PROSITE documentation number (PDOC00104).
- Check the databases SWISS-PROT, TrEMBL, TrEMBLnew, PDB and exclude splice variants.
- Click Start the Scan.
- Wait for the results.
Results:
- Scroll down the page and click Taxonomy tree view of hits. From here you can select the hits in Eukaryota. Click View only the selected taxon.
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| Additional Notes |
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