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Yes! However, as this is a eukaryotic sequence, the ORF finder will produce
results that are hard to interpret—remember, most eukaryotic genes consist of
Short coding regions (EXONS) with interspersed non-coding DNA (INTRONS),
among other features that may ”confuse“ the ORF Finder (try this with the FASTA
file of the region provided on your desktop to see the problem firsthand). Instead use
the GeneMachine, which is designed for this purpose-finding eukaryotic genes:
- Cut and paste the provided FASTA file of the chromosome region sequence data into the Web-based version of GeneMachine
at http://genemachine.nhgri.nih.gov/index.cgi, enter a valid e-mail address and then
click on ”Find Genes“.
- While waiting for the e-mail with the GeneMachine output, install Sequin on your
machine as per the instructions on the GeneMachine site.
- The resulting GeneMachine file (which is in ASN.1 format) as read in Sequin
indicates the existence of a single gene (FIG 2).
- BLASTing the sequence within that single gene region against the human genome
indicates that the sequence matches MLH1-the human version of MutL1 (FIG 3)…
FIG 2: GeneMachine Results
FIG 3: BLASTn results showing identity of gene
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