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Practice User Question: monoamine oxidase.

  Sample User Question
Analysis/Comments
Flow Chart
Step By Step Guide
  

Sample User Question back to top

Find genomic DNA clone that corresponds to the mRNA for (human) monoamine oxidase A.

Analysis/Comments back to top

This is a commonly asked user question. When a mRNA for a protein of interest has been identified, the researcher often wants to locate and examine the genomic DNA. Genomic DNA contains additional sequences of interest beyond the coding regions including the promoter site, transcription factor binding sites, intron sequences, and other sequences relevant to expression of the gene. Now that the nearly complete human genomic sequence is available, this type of question is becoming easy to answer. The textual genomic sequence is easily available and many of the BAC clones (in DNA form) used in the human genome sequencing project are now available via the Clone Registry from commercial and academic suppliers.

Flow Chart back to top

  1. Entrez Gene - Use Entrez Gene to view a summary of the available information on the human monoamine oxidase A gene. You can link from Entrez Gene to many other resources including the MapViewer.

  2. MapViewer - The MapView of the MAOA gene with Genes_Seq as the Master Map. You can use the Maps&Options link to change which maps are viewed.

Step By Step Guide back to top

  1. Search Entrez Gene for the gene of interest. Remember to add "AND human[orgn] to the Entrez Gene search to help narrow your search specifically to human genes.
  2. Note that you can link directly from the Entrez Gene record to the genomic sequence of the gene via clicking on the accession # for the X chromosome (NC_000023) in the "Genomic Context" section and clicking on FASTA. That allows you to download the genomic nucleotide sequence directly. To locate a DNA clone containing the gene sequence that could be available for laboratory use, you can go to the MapViewer using the link from the "Genomic Context" section.
  3. Once the MapViewer is open, use "Maps & Options" to add maps such as the component and clone maps.
  4. Examine the maps for a clone that contains the entire gene and click on its identifier number to get more information. Once a specific clone has been identified, the clone record may provide information on obtaining it from a supplier.

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