Demonstration Question: Using the Model Maker
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| Sample User Question
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Use the ModelMaker to construct a model for EDN1 that
excludes exon 3 and download the resulting sequence.
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| Analysis/Comments |
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The process of gene discovery and annotation is vital to our understanding
and productive use of the human genome sequence. There are estimated
to be approximately 30,000 human genes total. Some of these have yet
to be identified and many of the identified genes have yet to be located
and their structure and function fully determined. The identification
of genes in the MapViewer of the genome is based on an interpretation of the
available evidence accumulated in GenBank and other databases. As this
evidence is not always complete, the interpretation can be altered
as new evidence is added. The Model Maker allows the individual scientist
to re-examine the evidence and add to it, in order to make a new
model of a gene's structure.
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| Flow Chart |
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- MapViewer - This allows you
to view the maps of the human genome and the genomic context for genes, along with links to multiple
other NCBI databases.
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Model Maker - This allows you to view and manipulate the evidence for
the structure of a gene such as the locations of exons and introns.
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| Step By Step Guide |
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- View EDN1 in the MapViewer with the Genes_Seq map as the master map. Click on "mm" in the
annotation to go to the Model Maker.
- In the Model Maker's graphical exon view, click on each of the following exons to
select them: exon 1, exon 2, exon 4, exon 5 (excluding exon 3). This will
add the exon data to the text boxes.
- Click on the "save" link. Note that it will save the sequence in a FASTA
format. The resulting file is plain text and can be read with any textprocessing
program. From Netscape, it may try to put a .cgi extension on the file. Changing
that to .txt may be helpful. From IE, it will probably give the file a .fa
extension. That, also, can be altered to .txt.
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