DNA Analysis
Course Home Modules Schedule Exercises Comments Credits
Slide 1 Previous Next Slide List

What, Me Worry?

How Primer Design Can Affect PCR Outcomes.

  • Primer-Dimer formation
  • Secondary Structures in Primers
  • Illegitimate Priming in Template DNA due to repeated sequences
  • Incompatibility with PCR conditions

DNA Analysis
Slide 1 Previous Next Slide List
Revised 07/30/2002