Genetics Review
PubMed Entrez BLAST OMIM Taxonomy Structure
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Course Description

Schedule

Introduction

Genetics Review

Types of Databases

Format of Sequence Record

Entrez

BLAST

3-D Structures

Genomes and Maps

Librarian Roles

WWW Sites

Glossaries and Dictionaries

 

Radiation Hybrid Map back to top

  • GeneMap'99

  • use varying doses of radiation to break chromosome into pieces

    • GB4 RH panel:  3,000 Rads
      breaks DNA into approximately 10 megabase pieces
      average resolution of the map is 1 megabase
      scale of the GB4 panel is cR3000

    • G3 panel:  10,000 Rads
      breaks DNA into approximately 3 megabase pieces
      average resolution of the map is 500 kb
      scale of the G3 panel is cR10000

  • similar in ways to a genetic linkage map because the distance of two markers from each other is related to the frequency with which they break apart. The higher the frequency, the greater the distance. However, radiation hybrid maps have a higher resolution than genetic linkage maps.

More... back to top

Scale:  centiRay (cR)

CentiRays are not a constant unit, but the value of each centiray depends on the dose of radiation used to break the DNA into fragments.

E.g., If two genes are 1 centiRay apart (at a given dose of radiation), then there is a 1% chance of getting a break between them.

E.g., 1cR8000 = 1% chance that two things will break apart at a dose of 8000 rads.

Source:  The example above is from the glossary of:  Strachan, T. and Read, A.P. 1997. Human Molecular Genetics. New York: John Wiley & Sons.
Conversion of centiRay (cR) --> megabase (Mb) --> kilobase (Kb)

The average Kb length per CentiRay for the cR3000 and cR10000 panels can be estimated as follows, but keep in mind it provides an average value for the whole human genome, but the actual ratio can vary along portions of a chromosome:

GB4: Total cR3000 for the genome is 11524, so:
3,200 Mb/11,524 cR = 0.28 Mb/cR X 1000 Kb/Mb = 280 Kb/cR

G3: Total cR10000 for the genome is 125853, so:
3,200 Mb/125,853 cR = 0.025 Mb/cR X 1000 Kb/Mb = 25 Kb/cR

Help Desk NCBI NLM NIH Credits
  Revised September 29, 1999
Comments/questions about course to Renata Geer renata@ncbi.nlm.nih.gov
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