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Questions module of the MLA course on Introduction to Molecular Biology 
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User Questions Grab Bag

  1. How can I retrieve the previous version of the sequence data in accession U46667?

  2. Accession U67889 has a GC_signal, TATA_signal, 5' UTR, and 3'UTR annotated in the Feature field. What are those? Where can you find the answer?

  3. I'd like to retrieve mouse mRNA sequences for the huntington disease gene, but I don't want any ESTs in the results.

  4. I'd like to retrieve all protein sequences for the family Canidae. How can I do that?

  5. Database statistics: How many reviewed RefSeq records do we currently have for human proteins? How many provisional human RefSeq proteins are there?

  6. Database statistics: How many records are currently in the Entrez Structure database?

  7. When I BLASTed _____ against the nucleotide nr database, a number of the bases were replaced with NNNN's. Why did that happen, and how can I see my complete query sequence?

  8. What is the official gene symbol for the human cystic fibrosis gene, and by what other symbols has it been known?

  9. I'd like to retrieve the human and mouse sequences for the PSEN1 gene, and compare them against each other. What is an easy way to do that?

  10. How many protein chains combine with each other to make the P53 tumor suppressor protein that is shown in the structure record 1TUP? What is the accession number of the corresponding Entrez Protein record for each chain?

  11. What is the chromosomal location of the human Huntington Disease gene, both the cytogenetic band and the sequence coordinates? What is the corresponding location in mouse?

  12. I'd like to download the genomic sequence for the human HD gene, plus 1000 bp upstream and downstream. How can I do that?

  13. What sequence tagged sites (STSs) exist in the PSEN1 (presenilin-1) gene that is involved in Alzheimers Disease?

  14. What genes have been mapped to the the 3p21.3 band of the human genome?

  15. Where can I download the complete genome sequence for E. coli?

  16. What clinical information can I find for the MLH1 gene involved in colon cancer? What labs offer genetic tests for that disease?

  17. How can I find a restriction map of my sequence?

User Questions
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Revised 12/29/2004