NCBI Home GenBank Annotation Examples

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GENERAL REQUIREMENTS
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Examples

 mRNA sequence

 Prokaryotic gene

 Eukaryotic gene

 rRNA and/or ITS

 Promoter region

 Viral sequence

 HIV-1

 Transposon or insertion sequence

 Microsatellite

 Repeat regions

 Pseudogene

 Translocation and/or fusion protein

 Cloning vector

 Segmented set of exons/introns

 Phylogenetic or population studies

 EST submissions

 STS submissions

 GSS submissions

 HTGs

 FLICs

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  Cloning vector

Relevant feature information for a cloning vector
  • unique name for the cloning vector
Optional:
  • coding region intervals, including start and stop codons
  • protein names, gene names

We strongly suggest that you provide as much of the above information as possible to ensure the most complete annotation of your sequence. If any of this information is not known, please inform us.

Example:

Cloning vector pRB223, complete sequence

FEATURES             Location/Qualifiers

     source          1..4361
                     /organism="Cloning vector pRB223"
                     
     gene            86..1276
                     /gene="tet"
                     
     CDS             86..1276
                     /gene="tet"
                     /product="tetracycline resistance protein"

     RBS             1905..1909
                     /note="Shine-Dalgarno sequence"
                     
     rep_origin      2535
                     
     gene            complement(3293..4194)
                     /gene="bla"
                     
     CDS             complement(3293..4153)
                     /gene="bla"
                     /product="beta-lactamase"

     misc_feature    4069..4125
                     /note="multiple cloning site"

     RBS             complement(4161..4165)
                     /gene="bla"     
                     /note="Shine-Dalgarno sequence"

     promoter        complement(4188..4194)
                     /gene="bla"


 

Revised November 28, 2000