NCBI Home GenBank Annotation Examples

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GENERAL REQUIREMENTS
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Examples

 mRNA sequence

 Prokaryotic gene

 Eukaryotic gene

 rRNA and/or ITS

 Promoter region

 Viral sequence

 HIV-1

 Transposon or insertion sequence

 Microsatellite

 Repeat regions

 Pseudogene

 Translocation and/or fusion protein

 Cloning vector

 Gapped Sequence

 Phylogenetic or population studies

 EST submissions

 STS submissions

 GSS submissions

 HTGs

 FLICs

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  Microsatellite Sequence

Relevant feature information for a microsatellite sequence:
  • unique microsatellite/clone name for each sequence
  • interval of any repeat region(s) within the microsatellite sequence, if known
  • are these considered STS sequences?

We strongly suggest that you provide as much of the above information as possible to ensure the most complete annotation of your sequence. If any of this information is not known, please inform us.

Example #1:

Chorthippus parallelus clone IIB-G5 microsatellite sequence.

FEATURES             Location/Qualifiers

     source          1..288
                     /organism="Chorthippus parallelus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:37639"
                     /clone="IIB-G5"

     repeat_region   1..288
                     /rpt_type=tandem
                     /satellite="microsatellite"

Example #2:

Noturus exilis voucher KU 40271 microsatellite Noex254 sequence.

FEATURES             Location/Qualifiers

     source          1..556
                     /organism="Noturus exilis"
                     /mol_type="genomic DNA"
                     /specimen_voucher="KU 40271"
                     /db_xref="taxon:61323"
                     /clone="Noex_02_03_H06"
                     /PCR_primers="fwd_seq: catgtttgcacaaagggaaa, rev_seq:
                     atgtggatgcagattgtgga"

     repeat_region   77..100
                     /rpt_type=tandem
                     /rpt_unit_range=77..100
                     /rpt_unit_seq="ca"
                     /satellite="microsatellite:Noex254"


 

 

Revised June 2, 2009