NCBI Home GenBank Annotation Examples

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GENERAL REQUIREMENTS
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Examples

 mRNA sequence

 Prokaryotic gene

 Eukaryotic gene

 rRNA and/or ITS

 Promoter region

 Viral sequence

 HIV-1

 Transposon or insertion sequence

 Microsatellite

 Repeat regions

 Pseudogene

 Translocation and/or fusion protein

 Cloning vector

 Segmented set of exons/introns

 Phylogenetic or population studies

 EST submissions

 STS submissions

 GSS submissions

 HTGs

 FLICs

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  Ribosomal RNA and Internal Transcribed Spacers

Relevant feature information for a genomic sequence containing structural RNAs and/or spacers:
  • names of any structural RNAs (eg, tRNA-Ile, 16S ribosomal RNA)
  • names of any spacer regions (eg, internal transcribed spacer 1, 16S/23S intergenic spacer)
  • nucleotide spans of each of the above features, if known

We strongly suggest that you provide as much of the above information as possible to ensure the most complete annotation of your sequence. If any of this information is not known, please inform us.

Example:

Saccharomyces cerevisiae 18S ribosomal RNA gene, partial sequence;
internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal
transcribed spacer 2, complete sequence; and 28S ribosomal RNA
gene, partial sequence.

FEATURES             Location/Qualifiers

     source          1..540
                     /organism="Saccharomyces cerevisiae"
                     /strain="UMD 334"

     rRNA            <1..5
                     /product="18S ribosomal RNA"
                     
     misc_RNA        6..178
                     /product="internal transcribed spacer 1"
                     
     rRNA            179..377
                     /product="5.8S ribosomal RNA"
                     
     misc_RNA        378..519
                     /product="internal transcribed spacer 2"
                     
     rRNA            520..>540
                     /product="28S ribosomal RNA"


 

Revised November 28, 2000