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11/25 Electronic PCR Electronic PCR is now available. PCR-based sequence tagged sites (STSs) have been used as landmarks for construction of various types of genomic maps. Using "electronic PCR" (e-PCR), these sites can be detected in DNA sequences, potentially allowing their map locations to be determined.

11/14 Sequin, Release 1.71 A new release of Sequin, a sequence submission tool, is now available. Version 1.71 features improved handling of phylogenetic sets of sequences and also allows users to update their own pre-existing database records.

11/04 dbGSS Announced The Database of Genome Survey Sequences (dbGSS) is now available. This database contains more detailed information than the corresponding records in the GSS Division of GenBank.

10/24 Human Gene Map The Gene Map of the Human Genome published in the October 25 issue of Science is available. This map shows the chromosome location of over 16,000 human genes with links to the underlying sequence and map data.

10/04 Sequin Sequin, a stand-alone sequence submission tool, has a new release with several enhancements, including a repeat finder and ORF finder. New documentation and a tutorial are available, both on the Web and in NCBI's newsletter.

09/27 ORF Finder The Open Reading Frame (ORF) Finder is a graphical analysis tool that finds all open reading frames in a user's sequence or one already in the database of a selectable minimum size.

09/06 Virological  Software Software for analyzing animal trials and calculating infectious and 50% inhibitory doses is now available. The programs VacMan and ID-50 can now be downloaded as self-extracting archives for either IBM or Macintosh computers.

08/23 Complete Genome, Methanococcus jannaschii The complete genome sequence and annotation of Methanococcus jannaschii, prepared by The Institute for Genomic Research (TIGR) is now available in Entrez Genome, as well as in GenBank, where the 1.7-megabase sequence has been separated into 150 records of approximately 11,000 bp each. The graphical view (as well as a link to underlying data) of the complete genome is present in Entrez Genome, along with the extrachromosomal elements 1 and 2. The complete sequence is also available by anonymous FTP; see the README file for a description of the various files in the genomes division directory.

08/20 Batch Entrez Downloading large numbers of sequence records from Entrez is now possible through 'Batch Entrez'. User can specify a download for an entire set of records for a given organism or for a set of accession numbers. The data are saved to a file on the user's computer.

08/05 Saccharomyces cerevisiae Database A new database has been added to the BLAST databases: all the nucleotide sequences from the yeast (Saccharomyces cerevisiae) genome sequencing project and their encoding amino acid sequences can now be searched with the BLAST suite of programs.

07/26 Cn3D in Entrez A major new release of Network Entrez is now available. Release 5.0 contains Cn3D, a new 3D structure viewer integrated into Network Entrez.

07/15 BLAST2 The BLAST2 network service is now available on the FTP site without registration. Three clients for multiple platforms are available: blastcli has a convenient graphical interface and produces the "traditional" BLAST output; blastcl2 is a command-line client (meant mostly for UNIX) that also produces the traditional BLAST output; and PowerBlast produces a one-to-many alignment, allows filtering by organism, and allows a gapped alignment as a post-processing of the BLAST results. Users of the older Experimental BLAST Network Service (with the exception of GCG users, who are still required to register and use the older program) are encouraged to switch to this newest version.

05/21; see also 11/14 Sequin Sequin is a program for submitting and updating GenBank entries. It is designed to simplify the sequence submission process, provide graphical viewing and editing options, and allow submission of segmented entries. Sequin automatically adjusts feature table positions as the sequence is edited. Versions of Sequin are available through FTP for the Macintosh, PC/Windows, UNIX, and VMS.

05/21 PowerBlast PowerBlast is a new network BLAST application for automated analysis of genomic sequences. It combines BLAST searching with filtering for low complexity regions and repeats. It can generate organism-specific output and compute optimal, gapped alignments. The results are displayed graphically and textually as multiple alignments, with annotated features superimposed on the aligned sequences. Versions of PowerBlast are available through FTP for the Macintosh, PC, SunOS, and Solaris.

05/06 WWW BLAST The WWW BLAST page has been extensively revised. It now has both a simplified "Basic" Blast Search, allowing a user to search with the default parameters, as well as an "Advanced" page, where users may set BLAST parameters. An email option allows a user to receive results in a convenient form.

04/10

WWW Entrez

WWW Entrez now provides graphical views of nucleotide and protein sequences and access to the NCBI Genomes database, which contains graphical views of sequences and chromosome maps. Click on "Graphical view" from an Entrez document summary or click on the "Graphic" button from a sequence report.

03/12 Mouse/Human Homology The Seldin/Debry Mouse/Human Homology Relationships page presents a table comparing genes in homologous segments of DNA from human and mouse sources, sorted by position in each genome.

03/07 Complete Genomes An NCBI research project, Complete Genomes, presents the results of analyses of complete genome sequences. The analyses for the genomes of Haemophilus influenzae, E. coli (75%), and Mycoplasma genitalium are now available.

03/08 BLAST Databases Changes to the BLAST Databases (February 20 announcement superseded by that of March 8.)

02/15 Homepage Reorganization Major reorganization of the NCBI homepage with new top-level links to additional databases and services.

02/07 International Database Collaboration The International Nucleotide Sequence Database Collaboration page describes current projects and provides links to the sites.

01/30 NCBI Structure Group The NCBI Structure Group (Steve Bryant) has a new page providing access to their structure research, the PKB and MMDB databases, and threading software.


Revised: June 6, 2002.